|
KMID : 0903519950380050435
|
|
Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1995 Volume.38 No. 5 p.435 ~ p.439
|
|
|
Characteristics and Pathogenicity of Hyphantria cunea Nuclear Polyhedrosis Virus
|
|
|
|
|
Abstract
|
|
|
|
Some characteristics and pathogenicity of Hyphantria cunea nuclear polyhedrosis virus (HcNPV), a potential microbial pesticide was studied. H cunea NPV replicated in the nucleus of S. frugiperda cells cultured in the TNMFH medium. In case of virus infected cell, prepolyhedra formation was observed at 24 hrs post-infection. At 48 hrs post-infection. Most of the infected cell contained many mature polyhedra, which were released into culture media 72 hrs post-infection, with the cells grown in suspension culture, pH of the culture medium increased during the virus replication: the pH of fresh medium was 6.35 and rose to 6.77 within 120 hrs. Polyhedra formed a band in linear density gradient of sucrose by centrifugation, which co-sedimented with 50∼55% sucrose. The shape of the purified polyhedra was mostly tetragonal hexahedron and its size was about 2.5 ㎛. Electron microscopy and phase contrast microscopy showed that many bundled nucleocapsids were occluded in mature polyhedra at 48 hrs post infection. H. cunea larvae infected with NPV showed a higher motility in the second and third instar than in the fourth instar. Death rate of H. currea larvae in the second and third instar fed with leaves coated with 1.5×10^9∼1.5×10^7 PIBs/㎖ reached more than 90%.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|